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16100 pierce glutathione agarose Pierce™ Chromatography Cartridges 5 Cartridges, 1 mL Please select your glutathione flavor:Cacao Mint
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Learn more about photocleavable biotin peptides → Technical Considerations for Biotinylated Peptide Design Labeling position: choose N-terminal, C-terminal, or Lys side-chain biotinylation based on the active binding region Spacer length: use Ahx or PEG spacers when surface accessibility is important Peptide solubility: hydrophobic peptides may require sequence adjustment, PEG spacing, or solubility tags Assay orientation: avoid placing biotin too close to the binding motif if steric hindrance is possible Control design: include scrambled, mutant, unmodified, or non-biotinylated controls when interpreting binding results Additional modifications: biotin can often be combined with phosphorylation, acetylation, methylation, fluorescent labels, or other modifications Publication Examples Involving Biotin-Based Interaction Studies Biotin-based labeling and capture systems continue to support important studies in immunology, cell-cell interaction mapping, and peptide/protein interaction analysis
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