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Cell secretion mediated by granule-associated vesicle transport: A glimpse at evolution
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SIZE::120 ml / 4 oz
Cells were first washed with KHM buffer (110 mM potassium acetate, 20 mM HEPES, 2 mM MgCl2) for 2 min at 6 psi (41.4 kPa), then treated with 20 μM of digitonin for 3 min at 6 psi, and with 50 μg/ml proteinase K at 6 psi
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