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Detecting Oxidative Damage Oxidation indicators include: Mass spectrometry +16 Da, +32 Da shifts (oxygen addition) New HPLC peaks with earlier retention times Yellow to brown discoloration Reduced biological activity Increased UV absorbance at 280nm Free sulfhydryl group loss (for cysteine-containing peptides) Mistake #7: Extended Storage Beyond Stability Timelines Perhaps the most common mistake is simply storing peptides beyond their validated stability windows
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