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Western Blot Analysis of Hepatic Protein Expression Total protein was isolated from liver by homogenization in lysis buffer (0.5 M EDTA, 1 M Tris-HCl pH 8.1, 10% SDS, 10% Empigen,) with 1 × Complete Protease Inhibitor (Roche) and 1 × Phosphatase Inhibitor (Thermo Scientific, Waltham, MA, USA)
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