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To determine if Gclc- deficient Tregs increased glycolysis to produce the 3-PG needed for their enhanced serine production, we incubated Gclc- deficient and control iTregs for 24hr with the PHGDH inhibitor PKUMDL-WQ-2101 (Wang et al., 2017) and found equivalent decreases in ECAR (Figure 7A)